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Biotechnological approaches for plant viruses resistance: from general to the modern RNA silencing pathway BABT
Rodrigues,Silas Pessini; Lindsey,George G.; Fernandes,Patricia Machado Bueno.
Virus diseases are significant threats to modern agriculture and their control remains a challenge to the management of cultivation. The main virus resistance strategies are based on either natural resistance or engineered virus-resistant plants. Recent progress in understanding the molecular mechanisms underlying the roles of resistance genes has promoted the development of new anti-virus strategies. Engineered plants, in particular plants expressing RNA-silencing nucleotides, are becoming increasingly important and are likely to provide more effective strategies in future. A general discussion on the biotechnology of plant responses to virus infection is followed by recent advances in engineered plant resistance.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant defense; Engineered resistance; Phytopathogens.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400002
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Closely-related Xanthomonas citri subsp. citri isolates trigger distinct histological and transcriptional responses in Citrus limon Scientia Agricola
Orce,Ingrid Georgina; Debes,Mario; Sendín,Lorena; Luque,Ana; Arias,Marta; Vojnov,Adrián; Marano,María; Castagnaro,Atilio; Filippone,María Paula.
ABSTRACT Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), has an important economic impact on the citrus industry. Extensive information is available about the disease but, nevertheless, the study of plant-pathogen interactions could provide new information in the understanding of citrus canker disease. A new isolate has been identified, Xcc AT, which has a high genetic similarity (> 90 %) to the virulent Xcc T strain based on genetic clustering analyses of the rep-PCR fingerprinting patterns, but it does not produce cankerous lesions in Citrus limon. In this study, we compared C. limon responses to Xcc AT and to the virulent Xcc T strain at both histological and transcriptional levels. Histologically, leaves inoculated with Xcc AT...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CDNA-AFLP; Gene expression; Canker; Plant defense; Microscopy.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000600552
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Distribution pattern of herbivorous insects in a remnant of Brazilian Atlantic Forest Neotropical Entomology
Barbosa,Veralucia S.; Leal,Inara R.; Iannuzzi,Luciana; Almeida-Cortez,Jarcilene.
In this study we selected three habitats that correspond to a light gradient, and consequently a proportion of pioneer plant species gradient edges, trails and forest core and described their herbivorous insect assemblage. We tested five hypothesis about the herbivorous assemblage: (1) there is a different distribution of abundance and richness of order between habitats, (2) species similarity between habitats is smaller than within habitat, (3) in forest edge the herbivores are more abundant than in trails and interior of the forest, (4) in nuclear areas herbivore richness and diversity are higher than in the trails and edge, and (5) the abundance and richness of herbivores are different between seasons. Herbivorous were sampled during November 2000 - May...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fragmentation; Herbivorous insect assemblage; Herbivory; Insect diversity; Plant defense; Plant-insect interaction.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000500001
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Mecanismos de defesa de plantas contra o ataque de agentes fitopatogênicos. Infoteca-e
FERNANDES, C. de F.; VIEIRA JUNIOR, J. R.; SILVA, D. S. G. da; REIS, N. D.; ANTUNES JÚNIOR, H..
Este trabalho apresenta os principais aspectos envolvidos no mecanismo de defesa de plantas contra o ataque de patógenos, evidenciando a atuação de cada componente.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Plant defense; Resposta Hipersensitiva; Fitopatógeno.; Controle Biológico; Defesa Vegetal; Patógeno.; Plant pathogens; Hypersensitive response; Biological control..
Ano: 2009 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/710939
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Phytophagous mites on genetically modified maize with Bacillus thuringiensis genes Ciência Rural
Ferreira,Tamara Esteves; Fadini,Marcos Antonio Matiello; Mendes,Simone Martins; Marinho,Cidália Gabriela Santos; Cruz,Ivan.
ABSTRACT: The study of non-target organisms such as mites, can provide information about the possible effects of transgenic maize cultivars on the arthropod community. This study aimed to evaluate the abundance of phytophagous mites in the area of Bt maize and evaluate the instantaneous population growth rate, and food preference of phytophagous mites on Bt maize. We registered the species occurrence and the number of adult mite individuals on four maize cultivars, one non-Bt 30F35, and three containing the proteins Cry1Ab (30F35 Yg), Cry1F (30F35 Hx) and Vip3a (Impacto Viptera). Cry proteins disrupt the midgut epithelium of insect pests. The food preference and instantaneous population growth rate (ri) were evaluated using the two-spotted spider mite...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant defense; Non-target organisms; Genetically modified organisms Bt protein; Diptilomiopidae; Eriophyidae; Tetranychidae.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000202
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Polygala extraaxillaris: oxidative stress in Brachiaria decumbens mediated by volatile oils Planta Daninha
Silva,C.B.; Simionatto,E.; Gebara,S.S.; Poppi,N.R.; Cândido,A.C.S.; Lima,C.P.; Dias,J.F.G.; Zanin,S.M.W.; Peres,M.T.L.P.; Moreira,T.F.; Miguel,O.G.; Miguel,M.D..
The volatile oils extracted from the roots of Polygala extraaxillaris were analyzed to assess whether they increase oxidative stress in Brachiaria decumbens var. Piatã, as well as to assess their effect on cellular division and cytotoxicity in laboratory. Six concentrations were used (0%, 0.35%, 0.65%, 1.25%, 0.65%, and 5.0%) with four repetitions of 25 seeds. The substance 1-(2-hydroxyphenyl) - ethanone was identified as the major constituent of the volatile oils. The results showed that the highest concentrations of the oils resulted in an increase in the oxidative stress in B. decumbens, as well as alteration in germination and growth, with a consequent reduction in the process of cellular division, causing changes in the growth standard and antioxidant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reactive oxygen species; Antioxidant enzymes; Plant defense; Signal grass.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582013000400005
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Presence of the cytolytic protein enterolobin in different developmental stages of Enterolobium contortisiliquum seeds Braz. J. Plant Physiol.
Lima,Consuelo M. R. de; Zanotta,Pedro J. P.; Ricart,Carlos A. O.; Sousa,Marcelo V. de.
Enterolobin is a 52.9 kDa, cytolytic, inflammatory and insecticidal protein present in Enterolobium contortisiliquum (Vell.) Morong, a Brazilian large-sized tree. This protein was previously described to occur in different forms of association (monomeric and oligomeric forms). In the current work, we investigated the presence of enterolobin forms of association and hemolytic activity in maturing, germinating and germinated seeds. Immunoblotting analysis revealed the presence of the monomeric form of enterolobin in all phases under study, whereas the dimer was detected in developing seeds in the second half of the maturation period, during germination and in the early post-germination phase. The trimeric form of enterolobin appeared one week after the dimer...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Defense proteins; Hemolytic proteins; Plant defense; Seed germination.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000200008
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Purification and partial characterization of a lectin from Caesalpinia tinctoria Domb, ex Dc fruits Braz. J. Plant Physiol.
Oliveira,Marli Lourdes de; Beltramini,Leila Maria; Simone,Salvatore Giovanni de; Brumano,Maria Helena Nasser; Silva-Lucca,Rosemeire Aparecida; Nakaema,Marcelo Kiyoshi Kian; Pires,Christiano Vieira; Oliveira,Maria Goreti de Almeida.
A lectin was isolated from the pod saline extract of Caesalpinia tinctoria by dialoconcentration on Centripep-10 and affinity chromatography on chitin column. The purified lectin was partially characterized with respect to its biochemical and structural properties. It contains 8.3 % of carbohydrate and exhibited an agglutinating activity against human erythrocytes (ABO groups). Its amino acid composition was characterized by a great number of acidic and hydrophobic residues and the estimated molecular mass was 12.5 kDa. The presence of only one N-terminal amino acid sequence (D¹-V-P-A-Y-V-Y-V-H-F10-G-F-G-E-E-H-R -D-V-F20-D), showed the homogeneity of the purified lectin. The far-ultraviolet circular dichroism (CD) spectrum of lectin indicated that it...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Circular dichroism; Leguminosae; Plant defense; Pod; Secondary structure.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000200008
Registros recuperados: 8
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